Reference number
ISO 21115:2019
ISO 21115:2019
© ISO 2025
International Standard
ISO 21115:2019
Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1)
Edition 1
2019-04
2019-04
ISO 21115:2019
69933
Published
(Edition 1, 2019)
This publication was last reviewed and confirmed in 2024.
Therefore this version remains current.
Abstract
This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).
General information
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Status: PublishedPublication date: 2019-04Stage: International Standard confirmed [90.93]
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Edition: 1Number of pages: 38
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Technical Committee :ISO/TC 147/SC 5ICS :13.060.70
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